製品の概要

  • 製品名Anti-Glycophorin A antibody [YTH89.1]
    Glycophorin A 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [YTH89.1] to Glycophorin A
  • アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ・コントロール
    • IHC-P: Human hypophysis.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/25.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/1000.

This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

IHC-Fr 1/100 - 1/1000.

ターゲット情報

  • 機能Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • 配列類似性Belongs to the glycophorin A family.
  • 翻訳後修飾The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNacOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
  • 細胞内局在Cell membrane. Appears to be colocalized with SLC4A1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI853584 antibody
    • Blood group--MN locus antibody
    • CD_antigen=CD235a antibody
    • CD235a antibody
    • GLPA_HUMAN antibody
    • Glycophorin A (MNS blood group) antibody
    • Glycophorin A antibody
    • Glycophorin A, included antibody
    • Glycophorin-A antibody
    • GlycophorinA antibody
    • GPA antibody
    • GPErik antibody
    • GpMiIII antibody
    • GPSAT antibody
    • GYPA antibody
    • GYPA, included antibody
    • HGpMiIII antibody
    • HGpMiV antibody
    • HGpMiX antibody
    • HGpMiXI antibody
    • HGpSta(C) antibody
    • MN antibody
    • MN sialoglycoprotein antibody
    • MNS antibody
    • PAS-2 antibody
    • PAS2 antibody
    • Sialoglycoprotein alpha antibody
    see all

Anti-Glycophorin A antibody [YTH89.1] 画像

  • ab33386 staining erythrocytes in sinusoids of human hypophysis by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
  • ab33386 staining Glycophorin A in isolated human cord blood by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 1% BSA + 0.1% Triton X-100 + 0.05% Tween-20 + 0.01% NaN3. The sample was incubated with the primary antibody (2µg/ml in !% BSA + 0.05% Tween-20 + 0.01% NaN3) for 2 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rat IgG polyclonal (1/1000) was used as the secondary antibody.
    Gating Strategy: Against debris (low SSC/high FSC).

    See Abreview

Anti-Glycophorin A antibody [YTH89.1] (ab33386) 使用論文

This product has been referenced in:
  • Jokiranta TS & Meri S Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement. J Immunol 151:2124-31 (1993). Read more (PubMed: 7688394) »
  • Outram S  et al. Erythromyeloid lineage fidelity is conserved in erythroleukaemia. Leuk Res 12:651-7 (1988). Read more (PubMed: 3184981) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - 1% BSA, 0.1% Triton X-100, 0.05% Tween-20, 0.01% NaN3
Sample Human Cell (Isolated Human Cord Blood)
Specification Isolated Human Cord Blood
Gating Strategy Against debris (low SSC/high FSC)
Preparation Cell harvesting/tissue preparation method: 4% PFA fixed cells (12h), buffer washed (1% BSA, 0.05% Tween-20, 0.01% NaN3), permeabilised (buffer with 0.1% Triton X100), blocked (10% NGS)
Sample buffer: 1% BSA, 0.05% tween-20, 0.01% NaN3
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Mr. Mark Allenby

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投稿 Mar 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"