Anti-Glycogen synthase 2 抗体 (ab83550)


  • 製品名Anti-Glycogen synthase 2 antibody
  • 製品の詳細
    Rabbit polyclonal to Glycogen synthase 2
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: TLSRAFPDKF HVELTSPPTT EGFKYPRPSS VPPSPSGSQA SSPQSSDVED , corresponding to a region within internal sequence amino acids 612 - 661 of Human Glycogen synthase 2 (NP_068776)

  • ポジティブ・コントロール
    • Fetal muscle lysate



Our Abpromise guarantee covers the use of ab83550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 機能Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
  • パスウェイGlycan biosynthesis; glycogen biosynthesis.
  • 関連疾患Defects in GYS2 are the cause of glycogen storage disease type 0 (GSD0) [MIM:240600]; A metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood, high blood ketones and low alanine and lactate concentrations. Although feeding relieves symptoms, it often results in postprandial hyperglycemia and hyperlactatemia.
  • 配列類似性Belongs to the glycosyltransferase 3 family.
  • 翻訳後修飾Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC antibody
    • Glycogen [starch] synthase antibody
    • Glycogen starch synthase liver antibody
    • Glycogen synthase 2 liver antibody
    • Gys2 antibody
    • GYS2_HUMAN antibody
    • liver antibody
    see all

Anti-Glycogen synthase 2 antibody 画像

  • Anti-Glycogen synthase 2 antibody (ab83550) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
  • IHC image of ab83550 staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83550, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glycogen synthase 2 antibody (ab83550) 使用論文

ab83550 has not yet been referenced specifically in any publications.

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