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corresponding to Human Glycogen synthase 1.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Our Abpromise guarantee covers the use of ab81230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/2000. Predicted molecular weight: 85 kDa.|
|IHC-P||1/100 - 1/250.|
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Dot blot analysis of glycogen synthase 1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.