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RabMAb

Anti-Glycogen synthase 1 (phospho S641) 抗体 [EP852Y] (ab81230)

製品の概要

  • 製品名
    Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y]
    Glycogen synthase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP852Y] to Glycogen synthase 1 (phospho S641)
  • 特異性
    Detects Glycogen synthase 1 only when phosphorylated on Serine 641.
  • アプリケーション
    適用あり: Dot blot, WB, IHC-P, IPmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    corresponding to Human Glycogen synthase 1.

  • ポジティブ・コントロール
    • WB: Mouse muscle and Mouse muscle treated with AP lysates; HeLa and HeLa treated with insulin at 20 ug/mL for 15 minutes whole cell lysates. IHC: Human liver tissue.
  • 特記事項

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000.
WB 1/10000 - 1/2000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/250.
IP 1/60.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
    • パスウェイ
      Glycan biosynthesis; glycogen biosynthesis.
    • 関連疾患
      Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
    • 配列類似性
      Belongs to the glycosyltransferase 3 family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Glycogen [starch] synthase antibody
      • Glycogen synthase 1 (muscle) antibody
      • Glycogen synthase 1 antibody
      • GSY antibody
      • GYS antibody
      • Gys1 antibody
      • GYS1_HUMAN antibody
      • muscle antibody
      see all

    画像

    • All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230) at 1/2000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysates
      Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.
      Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates.Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000

      Predicted band size : 85 kDa
      Observed band size : 85 kDa


      Exposure time : 1 second

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    • All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilution

      Lane 1 : Mouse muscle lysate, untreated
      Lane 2 : Mouse muscle lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 85 kDa
      Observed band size : 85 kDa
    • Dot blot analysis of glycogen synthase 1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230.

    参考文献

    This product has been referenced in:
    • Zhang JS  et al. Differential activity of GSK-3 isoforms regulates NF-?B and TRAIL- or TNFa induced apoptosis in pancreatic cancer cells. Cell Death Dis 5:e1142 (2014). WB . Read more (PubMed: 24675460) »
    • Gao J  et al. Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle. BMC Complement Altern Med 14:198 (2014). WB . Read more (PubMed: 24952587) »

    See all 5 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycin gel)
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 28 2013

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Username

    Dr. Karine Thibault

    Verified customer

    投稿 Nov 17 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (gel 10% SDS-PAGE)
    Sample
    Mouse Tissue lysate - whole (substantia nigra)
    Specification
    substantia nigra
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Karine Thibault

    Verified customer

    投稿 Mar 05 2015

    I apologize for the delays due to retesting of this antibody.

    Lab has confirmed observing a band at 85kDa in human, rat and mouse muscle lysates. They have provided an image which clearly show the antibody is detecting a correct band at 85kDa...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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