Anti-Glycerol kinase 抗体 (ab88505)

製品の概要

  • 製品名Anti-Glycerol kinase antibody
    Glycerol kinase 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Glycerol kinase
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-524 of Human Glycerol kinase (NP_000158.1)

  • ポジティブ・コントロール
    • human placenta lysate, transfected 293T cell line, human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 57 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Key enzyme in the regulation of glycerol uptake and metabolism.
    • 組織特異性Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
    • パスウェイPolyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
    • 関連疾患Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.
    • 配列類似性Belongs to the FGGY kinase family.
    • 細胞内局在Mitochondrion outer membrane. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3 phosphotransferase antibody
      • ATP:glycerol 3-phosphotransferase antibody
      • D930012N15Rik antibody
      • GK antibody
      • GK1 antibody
      • GKD antibody
      • GLPK_HUMAN antibody
      • Glycerokinase antibody
      • Glycerol kinase antibody
      • OTTHUMP00000023108 antibody
      • OTTHUMP00000023109 antibody
      • OTTHUMP00000215321 antibody
      see all

    Anti-Glycerol kinase antibody 画像

    • Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml + human placenta lysate at 50 µg

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • All lanes : Anti-Glycerol kinase antibody (ab88505) at 5 µg/ml

      Lane 1 : transfected 293T cell line
      Lane 2 : non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      HRP goat anti mouse IgG at 1/2500 dilution

      Predicted band size : 57 kDa
    • Immunohistochemical analysis of Glycerol kinase expression in formalin-fixed paraffin-embedded human kidney using 3ug ab88505.

    Anti-Glycerol kinase antibody (ab88505) 使用論文

    ab88505 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
    Antigen retrieval step Heat mediated
    Sample Mouse Tissue sections (Adipose tissue)
    Specification Adipose tissue
    Permeabilization Yes - PBS 0.5% TritonX-100
    Fixative Paraformaldehyde
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    投稿 Dec 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"