Anti-Glutathione Synthetase 抗体 (ab91591)


  • 製品名Anti-Glutathione Synthetase antibody
    Glutathione Synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Synthetase
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of Human Glutathione Synthetase.

  • ポジティブ・コントロール
    • Mouse liver tissue lysate



Our Abpromise guarantee covers the use of ab91591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/1000.


  • パスウェイSulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
  • 関連疾患Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
    Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
  • 配列類似性Belongs to the eukaryotic GSH synthase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • epididymis secretory sperm binding protein Li 64p antibody
    • epididymis secretory sperm binding protein Li 88n antibody
    • Glutathione synthase antibody
    • Glutathione synthetase antibody
    • GSH S antibody
    • GSH synthetase antibody
    • GSH-S antibody
    • GSHB_HUMAN antibody
    • GSHS antibody
    • GSS antibody
    • HEL-S-64p antibody
    • HEL-S-88n antibody
    • MGC14098 antibody
    • OTTHUMP00000030711 antibody
    see all

Anti-Glutathione Synthetase antibody 画像

  • Anti-Glutathione Synthetase antibody (ab91591) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 52 kDa
  • IHC image of ab91591 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91591, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glutathione Synthetase antibody (ab91591) 使用論文

This product has been referenced in:
  • Scheving R  et al. Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve. J Proteomics 75:3987-4004 (2012). WB, ELISA ; Mouse . Read more (PubMed: 22588120) »

See 1 Publication for this product

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Please find the ELISA Protocol below.

Note: The precise conditions should be optimized for a particular assay.

Solution Preparation

Coating Solution:

Antigen is diluted in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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投稿 Aug 23 2011