Anti-Glutathione Synthetase 抗体 (ab91591)

製品の概要

  • 製品名
    Anti-Glutathione Synthetase antibody
    Glutathione Synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Synthetase
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of Human Glutathione Synthetase.

  • ポジティブ・コントロール
    • Mouse liver tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/1000.

ターゲット情報

  • パスウェイ
    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
  • 関連疾患
    Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) [MIM:266130]; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
    Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia.
  • 配列類似性
    Belongs to the eukaryotic GSH synthase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • epididymis secretory sperm binding protein Li 64p antibody
    • epididymis secretory sperm binding protein Li 88n antibody
    • Glutathione synthase antibody
    • Glutathione synthetase antibody
    • GSH S antibody
    • GSH synthetase antibody
    • GSH-S antibody
    • GSHB_HUMAN antibody
    • GSHS antibody
    • GSS antibody
    • HEL-S-64p antibody
    • HEL-S-88n antibody
    • MGC14098 antibody
    • OTTHUMP00000030711 antibody
    see all

画像

  • Anti-Glutathione Synthetase antibody (ab91591) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 52 kDa
  • IHC image of ab91591 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91591, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:
  • Choi WG  et al. eIF2a phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet. Nutr Metab (Lond) 14:48 (2017). Read more (PubMed: 28781602) »
  • Scheving R  et al. Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve. J Proteomics 75:3987-4004 (2012). WB, ELISA ; Mouse . Read more (PubMed: 22588120) »

See all 2 Publications for this product

レビューと Q&A

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Please find the ELISA Protocol below.

Note: The precise conditions should be optimized for a particular assay.

Solution Preparation

Coating Solution:

Antigen is diluted in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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