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RabMAb

Anti-Glutathione S Transferase mu 抗体 [EPR1936(2)] (ab108524)

製品の概要

  • 製品名
    Anti-Glutathione S Transferase mu antibody [EPR1936(2)]
    Glutathione S Transferase mu 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1936(2)] to Glutathione S Transferase mu
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Glutathione S Transferase mu aa 100-200. The exact sequence is proprietary.
    (Peptide available as ab180487)

  • ポジティブ・コントロール
    • WB: HeLa, HepG2, SH-SY5Y and NIH/3T3 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.Can be blocked with Glutathione S Transferase mu peptide (ab180487).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能
      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
    • 組織特異性
      Liver (at protein level).
    • 配列類似性
      Belongs to the GST superfamily. Mu family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Glutathione S alkyltransferase antibody
      • Glutathione S aralkyltransferase antibody
      • Glutathione S aryltransferase antibody
      • Glutathione S transferase M1 antibody
      • Glutathione S transferase mu 1 antibody
      • Glutathione S transferase mu antibody
      • Glutathione S-transferase Mu 1 antibody
      • GST class mu 1 antibody
      • GST class Mu antibody
      • GST class-mu 1 antibody
      • GST HB subunit 4 antibody
      • GST mu antibody
      • GST1 antibody
      • GSTM1 1 antibody
      • Gstm1 antibody
      • GSTM1-1 antibody
      • GSTM1_HUMAN antibody
      • GSTM1a 1a antibody
      • GSTM1a-1a antibody
      • GSTM1b 1b antibody
      • GSTM1b-1b antibody
      • GTH4 antibody
      • GTM1 antibody
      • H B antibody
      • HB subunit 4 antibody
      • MGC26563 antibody
      • MU 1 antibody
      • MU antibody
      • S (hydroxyalkyl)glutathione lyase antibody
      see all

    画像

    • All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SH-SY5Y whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + NIH/3T3 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/10000 dilution (purified) + PC-12 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 30 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Glutathione S Transferase mu antibody [EPR1936(2)] (ab108524) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa

    プロトコール

    参考文献

    ab108524 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 18 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 18 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15% Gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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