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Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
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Glutathione Reductase (GR, EC 126.96.36.199) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
|Catalase (lyophilized)||Clear||1 x 1ng|
|DTNB (lyophilized)||Red||1 vial|
|GR Assay Buffer||NM||1 x 100ml|
|GR Positive Control (10 mU; lyophilized)||Green||1 vial|
|GSSG (lyophilized)||Yellow||1 vial|
|Hydrogen peroxide||Orange||1 x 1ml|
|NADPH-GNERAT™ (lyophilized)||Blue||2 vials|
|TNB Standard (2.5 µmol)||Brown||1 x 1ng|
Our Abpromise guarantee covers the use of ab83461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).
Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)
Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.
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