Glutathione Reductase (GR) Assay Kit (ab83461)

製品の概要

  • 製品名Glutathione Reductase (GR) Assay Kit
  • テスト
    200 x 1 assay
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • アッセイタイプEnzyme activity
  • 検出感度
    > 0.1 mU/ml
  • 検出範囲
    0.1 mU/ml - 40 mU/ml
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, ?max = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
  • 細胞内局在Cytoplasmic and Mitochondrial
  • 別名
    • EC 1.8.1.7
    • Glutathione reductase mitochondrial
    • GR
    • GSR
    • MGC78522
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab83461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Glutathione Reductase (GR) Assay Kit 画像

  • Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Glutathione Reductase assay time line using ab83461.
  • TNB Standard Curve using ab83461.

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Glutathione Reductase (GR) Assay Kit (ab83461) 使用論文

ab83461 has not yet been referenced specifically in any publications.

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The procedure is given in protocol booklet.

http://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ...

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Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use ar...

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Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"