Anti-Glutathione Reductase 抗体 (ab84963)

製品の概要

  • 製品名Anti-Glutathione Reductase antibody
    Glutathione Reductase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Reductase
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, African Green Monkey
  • 免疫原

    Synthetic peptide within residues: PKPTIEVSGK KYTAPHILIA TGGMPSTPHE SQIPGASLGI TSDGFFQLEE , corresponding to internal sequence amino acids 180-229 of Human Glutathione Reductase (NP_000628)

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

ターゲット情報

Anti-Glutathione Reductase antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Glutathione Reductase with ab84963 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Glutathione Reductase. Right - Merge.
  • Anti-Glutathione Reductase antibody (ab84963) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Gel concentration: 12%

Anti-Glutathione Reductase antibody (ab84963) 使用論文

ab84963 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HCT116 colon cancer cell line)
Loading amount 60 µg
Specification HCT116 colon cancer cell line
Treatment different treatments
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"