製品の概要

  • 製品名
    Anti-Glutathione Reductase antibody
    Glutathione Reductase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Reductase
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, African green monkey
  • 免疫原

    Synthetic peptide within residues: PKPTIEVSGK KYTAPHILIA TGGMPSTPHE SQIPGASLGI TSDGFFQLEE , corresponding to internal sequence amino acids 180-229 of Human Glutathione Reductase (NP_000628)

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

ターゲット情報

Anti-Glutathione Reductase antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Glutathione Reductase with ab84963 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Glutathione Reductase. Right - Merge.
  • Anti-Glutathione Reductase antibody (ab84963) at 1 µg/ml (5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Gel concentration: 12%

Anti-Glutathione Reductase antibody (ab84963) 使用論文

This product has been referenced in:
  • Hamilton TR  et al. Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm. Oxid Med Cell Longev 2016:1687657 (2016). Read more (PubMed: 26881013) »
  • Joyce NC  et al. Age-related gene response of human corneal endothelium to oxidative stress and DNA damage. Invest Ophthalmol Vis Sci 52:1641-9 (2011). Read more (PubMed: 21087955) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (HCT116 colon cancer cell line)
Loading amount
60 µg
Specification
HCT116 colon cancer cell line
Treatment
different treatments
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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