Anti-Glutathione Reductase 抗体 (ab16801)

製品の概要

  • 製品名Anti-Glutathione Reductase antibody
    Glutathione Reductase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Reductase
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein purified from E.coli (Human).

  • ポジティブ・コントロール
    • HeLa or Jurkat whole cell lysate. Mouse or Rat lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/400.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000. Predicted molecular weight: 58 kDa.

ターゲット情報

Anti-Glutathione Reductase antibody 画像

  • ICC/IF image of ab16801 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16801, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • Predicted band size : 58 kDa
    ab16801 at a 1/2000 dilution staining approx. 58kDa Glutathione peroxidase in 1) HeLa cell lysate, 2) Jurkat cell lysate, 3) Mouse lung kysates and 4) Rat lung lysate by Western blot (ECL). ab16801 at a 1/2000 dilution staining approx. 58kDa Glutathione reductase in 1) HeLa cell lysate, 2) Jurkat cell lysate, 3) Mouse lung kysates and 4) Rat lung lysate by Western blot (ECL).
  • ab16801 staining Glutathione Reductase in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 15 hours at 4°C. ab6721 (1/500) was used as the secondary antibody.

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  • Ab6801 staining Human normal placenta. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Glutathione Reductase antibody (ab16801) 使用論文

This product has been referenced in:
  • Maher TJ  et al. ATP-binding cassette transporter Abcg2 lineage contributes to the cardiac vasculature after oxidative stress. Am J Physiol Heart Circ Physiol 306:H1610-8 (2014). WB . Read more (PubMed: 24727496) »
  • Trevisan R  et al. Zinc causes acute impairment of glutathione metabolism followed by coordinated antioxidant defenses amplification in gills of brown mussels Perna perna. Comp Biochem Physiol C Toxicol Pharmacol 159:22-30 (2014). IF ; Other . Read more (PubMed: 24095941) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Antigen retrieval step None
Permeabilization No
Specification kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 15 2016

Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Bone tissue (Tibia))
Specification Bone tissue (Tibia)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Helder Fonseca

Verified customer

投稿 Oct 06 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 32°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Jan 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

投稿 Nov 30 2011

I have processed your request to receive ab106843 as a replacement for ab16801. I have also sent ab97055, goat polyclonal to rat (Biotin) aswell. The ab106843 is backordered until December 12th though and so your order will ship as soon as it comes in...

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Thank you for your reply. I would be happy to send you ab106843 as a replacement. The secondary antibody that you originally ordered was an anti-rabbit, not an anti-mouse, is that correct? I would also be happy to send you ab97055 as well. ...

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Thank you for your reply. The antibody ab55075 has not been proven to work on mouse tissue, there is a chance that the antibody will work on mouse tissue but we cannot guarantee it. The only other antibody in our catalogue that will work on mouse tissu...

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I am sorry that you are having problems using ab16801 in IHC-P and in western blot. Normally I would try and offer protocol tips for the IHC-P staining but the fact that you are not getting a signal when using the antibody in western blot does show tha...

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The antibodies should be stable at room temperature for that short period of time. It at all possible though, if you can even keep them with an ice pack that would be better. If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"