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RabMAb

Anti-Glutathione Peroxidase 4 抗体 [EPNCIR144] (ab125066)

製品の概要

  • 製品名
    Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
    Glutathione Peroxidase 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPNCIR144] to Glutathione Peroxidase 4
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Glutathione Peroxidase 4. The exact sequence is proprietary.
    Database link: O70325

  • ポジティブ・コントロール
    • WB: Mouse testis, rat testis, human testis, human fetal liver, and human seminoma tissue lysates, LnCaP, Jurkat and HepG2 cell lysates. ICC/IF: HEK293 cells. IHC-P: Human kidney and stomach tissues. Flow Cyt: HeLa cells.
  • 特記事項

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/400.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/200.

ターゲット情報

  • 機能
    Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • 組織特異性
    Present primarily in testis.
  • 配列類似性
    Belongs to the glutathione peroxidase family.
  • 細胞内局在
    Mitochondrion. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glutathione peroxidase 4 antibody
    • GPX 4 antibody
    • GPX-4 antibody
    • GPX4 antibody
    • GPX4_HUMAN antibody
    • GSHPx-4 antibody
    • MCSP antibody
    • mitochondrial antibody
    • PHGPx antibody
    • Phospholipid hydroperoxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
    • snGPx antibody
    • snPHGPx antibody
    • Sperm nucleus glutathione peroxidase antibody
    see all

画像

  • Immunofluorescence staining of HEK293 cells with purified ab125066 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab125066 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Unpurified ab125066, at a 1/100 dilution, staining Glutathione Peroxidase 4 in paraffin-embedded Human kidney tissue by immunohistochemistry.

  • All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)

    Lane 1 : mouse testis lysate
    Lane 2 : rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Glutathione Peroxidase 4 (red) with ab125066 at a 1/400 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/20000 dilution (purified)

    Lane 1 : human fetal liver lysate
    Lane 2 : Jurkat lysate

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human stomach with purified ab125066 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Unpurified ab125066 staining Glutathione Peroxidase 4 in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Nuclear staining was carried out with DAPI.

    See Abreview

  • Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 22 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution (unpurified)

    Lane 1 : Human testis lysate
    Lane 2 : Human seminoma lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : Human fetal liver lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 22 kDa

参考文献

This product has been referenced in:
  • Luna-Sánchez M  et al. CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome. EMBO Mol Med 9:78-95 (2017). WB ; Mouse . Read more (PubMed: 27856619) »
  • Kagan VE  et al. Oxidized arachidonic and adrenic PEs navigate cells to ferroptosis. Nat Chem Biol 13:81-90 (2017). Read more (PubMed: 27842066) »

See all 11 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Loading amount
20 µg
Treatment
cystine deficient medium, glutamine high medium 48h
Specification
head and neck cancer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 02 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
No
Fixative
Methanol
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 05 2014

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde der ab125066 Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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