Anti-Glutathione Peroxidase 4 抗体 (ab41787)

製品の概要

  • 製品名Anti-Glutathione Peroxidase 4 antibody
    Glutathione Peroxidase 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Peroxidase 4
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Glutathione Peroxidase 4.

    (Peptide available as ab41786.)

  • ポジティブ・コントロール
    • ab41787 gave a positive signal in the following human tissue lysates: Brain, Testis, Heart, Skeletal Muscle, Kidney, Colon, Liver, Small Intestine (data not shown for Liver and Small Intestine).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
  • 組織特異性Present primarily in testis.
  • 配列類似性Belongs to the glutathione peroxidase family.
  • 細胞内局在Mitochondrion. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glutathione peroxidase 4 antibody
    • GPX 4 antibody
    • GPX-4 antibody
    • GPX4 antibody
    • GPX4_HUMAN antibody
    • GSHPx-4 antibody
    • MCSP antibody
    • mitochondrial antibody
    • PHGPx antibody
    • Phospholipid hydroperoxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase antibody
    • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
    • snGPx antibody
    • snPHGPx antibody
    • Sperm nucleus glutathione peroxidase antibody
    see all

Anti-Glutathione Peroxidase 4 antibody 画像

  • All lanes : Anti-Glutathione Peroxidase 4 antibody (ab41787) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)
    Lane 6 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 27 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab41787 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41787, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-Glutathione Peroxidase 4 antibody (ab41787) 使用論文

ab41787 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"