Anti-Glutathione Peroxidase 4 抗体 [7A4] (ab16737)

製品の概要

  • 製品名Anti-Glutathione Peroxidase 4 antibody [7A4]
    Glutathione Peroxidase 4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7A4] to Glutathione Peroxidase 4
  • アプリケーション適用あり: ELISA, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein purified from E.coli (Human).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
IP
ICC/IF
  • 追加情報ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml.
    WB: 1/2000. Predicted molecular weight: 22 kDa. NOTE: Only recombinant protein is detected by the antibody when Western blotting.
    ICC/IF: Use at a concentration of 1 - 5 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.
    • 組織特異性Present primarily in testis.
    • 配列類似性Belongs to the glutathione peroxidase family.
    • 細胞内局在Mitochondrion. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Glutathione peroxidase 4 antibody
      • GPX 4 antibody
      • GPX-4 antibody
      • GPX4 antibody
      • GPX4_HUMAN antibody
      • GSHPx-4 antibody
      • MCSP antibody
      • mitochondrial antibody
      • PHGPx antibody
      • Phospholipid hydroperoxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase antibody
      • Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody
      • snGPx antibody
      • snPHGPx antibody
      • Sperm nucleus glutathione peroxidase antibody
      see all

    Anti-Glutathione Peroxidase 4 antibody [7A4] 画像

    • ICC/IF image of ab16737 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16737, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-Glutathione Peroxidase 4 antibody [7A4] (ab16737) 使用論文

    ab16737 has not yet been referenced specifically in any publications.

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    I am sorry these products did not perform as stated on the datasheets and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number *****. To check the status of the order please contact our C...

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    Thank you for your enquiry and patience. I contacted the originator of ab16737 and they informed me that only recombinant protein is detected by this antibody when Western blotting. Thus, we think this is the reason that you are not seeing a signal wit...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"