Anti-Glutathione Peroxidase 2/GPX2 抗体 (ab64322)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 2/GPX2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glutathione Peroxidase 2/GPX2 antibody
Glutathione Peroxidase 2/GPX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glutathione Peroxidase 2/GPX2 -
由来種
Rabbit -
特異性
Reacts specifically with human 22 kDa Glutathione Peroxidase 2/GPX2 protein
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アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Glutathione Peroxidase 2/GPX2.
Database link: P18283 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/1000.
|
特記事項 |
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IHC-P
1/1000. |
ターゲット情報
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機能
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. -
組織特異性
Mostly in liver and gastrointestinal tract, not found in heart or kidney. -
配列類似性
Belongs to the glutathione peroxidase family. -
細胞内局在
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 2877 Human
- Entrez Gene: 14776 Mouse
- Entrez Gene: 29326 Rat
- Omim: 138319 Human
- SwissProt: P18283 Human
- SwissProt: Q9JHC0 Mouse
- SwissProt: P83645 Rat
- Unigene: 2704 Human
see all -
別名
- Gastrointestinal glutathione peroxidase antibody
- GI GPx antibody
- Glutathione peroxidase 2 (gastrointestinal) antibody
see all
画像
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Ab64322 staining human normal bladder tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab64322 は 3 報の論文で使用されています。
- Chavatte L et al. Elemental and molecular imaging of human full thickness skin after exposure to heavy metals. Metallomics 12:1555-1562 (2020). PubMed: 32851388
- Cecchini MJ et al. CDX2 and Muc2 immunohistochemistry as prognostic markers in stage II colon cancer. Hum Pathol 90:70-79 (2019). PubMed: 31121192
- Liu T et al. GPX2 overexpression indicates poor prognosis in patients with hepatocellular carcinoma. Tumour Biol 39:1010428317700410 (2017). PubMed: 28635398