Anti-Glutathione Peroxidase 1 抗体 [EPR3311] (ab108429)


  • 製品名Anti-Glutathione Peroxidase 1 antibody [EPR3311]
    Glutathione Peroxidase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3311] to Glutathione Peroxidase 1
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: P07203

  • ポジティブ・コントロール
    • Human fetal liver, SH SY5Y, and THP1 cell lysates; Human breast carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab108429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    Anti-Glutathione Peroxidase 1 antibody [EPR3311] 画像

    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
      Lane 3: THP1 cell lysate (20 µg)
      Lane 4: HL60 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab108429, observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108429 was shown to specifically react with Glutathione Peroxidase 1 when Glutathione Peroxidase 1 knockout samples were used. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab108429 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 22 kDa
    • ab108429, at 1/100 dilution, staining Glutathione Peroxidase 1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

    Anti-Glutathione Peroxidase 1 antibody [EPR3311] (ab108429) 使用論文

    This product has been referenced in:
    • Baqader NO  et al. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res 13:4398-423 (2014). WB ; Human . Read more (PubMed: 25133973) »

    See 1 Publication for this product

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