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Our Abpromise guarantee covers the use of ab50427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|ELISA||Use at an assay dependent concentration. Antibody detection limit dilution 1:32,000.|
ab50427 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining shows staining of cytoplasm in PCT epithelial cells.
ab50437 at 3µg/ml staining Glutathione Perxidase 1 in human cerebral cortex by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for staining.
ab50427 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"