製品の概要

  • 製品名
    Anti-Glutathione Peroxidase 1 antibody
    Glutathione Peroxidase 1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Glutathione Peroxidase 1
  • 特異性
    This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Guinea pig, Chimpanzee, Macaque monkey, Common marmoset
  • 免疫原

    Synthetic peptide:

    C-REALPAPSDDATA

    , corresponding to internal sequence amino acids 128-140 of Human Glutathione Peroxidase 1

  • ポジティブ・コントロール
    • Human Liver and kidney lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA Use at an assay dependent concentration. Antibody detection limit dilution 1:32,000.

ターゲット情報

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  • ab50427 (20µg/ml) staining of PFA-perfused cryosection of Porcin Kidney following microwave antigen retrieval with citrate buffer pH 3 and CY3-staining shows staining of cytoplasm in PCT epithelial cells.

  • Anti-Glutathione Peroxidase 1 antibody (ab50427) at 1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab50437 at 3µg/ml staining Glutathione Perxidase 1 in human cerebral cortex by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. The AP staining procedure was used for staining.

参考文献

This product has been referenced in:
  • Joyce NC  et al. Age-related gene response of human corneal endothelium to oxidative stress and DNA damage. Invest Ophthalmol Vis Sci 52:1641-9 (2011). Read more (PubMed: 21087955) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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