Anti-Glutathione Peroxidase 1 抗体 (ab22604)

製品の概要

  • 製品名Anti-Glutathione Peroxidase 1 antibody
    Glutathione Peroxidase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutathione Peroxidase 1
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Glutathione Peroxidase 1.

    (Peptide available as ab25301.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human, Rat and mouse liver tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Glutathione Peroxidase 1 antibody 画像

  • ab22604 staining mouse Cor-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with TBS/BSA/azide/0.1%Tween 20 and blocked with 1% BSA for 10 minutes at RT. Samples were incubated with primary antibody (1/300) for 2 hours. An diluted (1/1000) Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview



  • Predicted band size : 22 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: GPX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: THP1 cell lysate (20 µg)
    Lane 4: HL60 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab22604 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab22604 was shown to recognize Glutathione Peroxidase 1 when Glutathione Peroxidase 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Peroxidase 1 knockout samples were subjected to SDS-PAGE. ab22604 at a concentration of 1µg/ml and ab8245 (loading control to GAPDH) diluted at 1/2000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Image courtesy of Human Protein Atlas

    ab22604 staining Glutathione Peroxidase 1 in Human kidney. The paraffin embedded human kidney tissue was incubated with ab22604 (1/375 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab22604 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab22604 stained human HepG2 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab22604, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/ml (Blocked with 3% Milk)

    Lane 1 : Liver (Human) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 190 kDa,55 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab22604 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-Glutathione Peroxidase 1 antibody (ab22604) at 1 µg/ml

    Lane 1 : Human liver normal tissue lysate
    Lane 2 : Liver (Mouse) Tissue Lysate (ab7935)
    Lane 3 : Human liver normal tissue lysate with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/ml
    Lane 4 : Liver (Mouse) Tissue Lysate (ab7935) with Human Glutathione Peroxidase 1 peptide (ab25301) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 22,24 kDa (why is the actual band size different from the predicted?)

    ab22604 detects a band of approximately 22 kDa in human liver lysate. The band detected in the mouse liver lysate runs slightly higher (~ 24kDa). We believe that these bands correspond to Glutathione Peroxidase 1 as they are both blocked by the addition of the immunizing peptide (ab25301).

    See Abreview

Anti-Glutathione Peroxidase 1 antibody (ab22604) 使用論文

This product has been referenced in:
  • Crilly MJ  et al. The role of Nrf2 in skeletal muscle contractile and mitochondrial function. J Appl Physiol (1985) 121:730-40 (2016). Read more (PubMed: 27471236) »
  • Di Filippo C  et al. Daily Oxygen/O3 Treatment Reduces Muscular Fatigue and Improves Cardiac Performance in Rats Subjected to Prolonged High Intensity Physical Exercise. Oxid Med Cell Longev 2015:190640 (2015). WB ; Rat . Read more (PubMed: 26265981) »

See all 27 Publications for this product

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It does appear that there is a problem with the vial of ab22604 you received. As you have purchased this antibody within the last 6 months I would be happy to offer a replacement or credi...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (ovarian cancer (OVCAr-3))
Permeabilization Yes - Ethanol 70%, 30 min at -20
Specification ovarian cancer (OVCAr-3)
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Abcam user community

Verified customer

投稿 Nov 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Antigen retrieval step None
Permeabilization No
Specification kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 15 2016

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

投稿 Feb 23 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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Verified customer

投稿 Feb 18 2016

Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (Tibia)
Specification Tibia
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Mr. Helder Fonseca

Verified customer

投稿 Sep 25 2014

The Lab has confirmed that they haven't done any specific tests to distinguish cellular localizations yet. However, it is likely that it would detect both forms. At the moment, I am unable to share more specific data with you regarding isoform specific...

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Ich habe unseren Produktkatalog für sie durchsucht und möchte Ihnen nun ein Angebot machen. Wir haben leider zur Zeit nur einen AGE Antikörper im Angebot und dieser ist unserem Wissen nach noch...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle-AT)
Loading amount 50 µg
Specification Skeletal muscle-AT
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

投稿 Sep 28 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"