Anti-Glutathione Peroxidase 1 抗体 (ab21966)

製品の概要

  • 製品名
    Anti-Glutathione Peroxidase 1 antibody
    Glutathione Peroxidase 1 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Glutathione Peroxidase 1
  • アプリケーション
    適用あり: Dot blot, IHC-P, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    RRYSRRFQTIDIEPDIEALL

    , corresponding to C terminal amino acids 175-194 of Human Glutathione Peroxidase

  • 特記事項


    ab21966 reacts with cellular glutathione peroxidase. This is a 201 amino acid selenium dependant cytoplasmic enzyme that exists as a homotetramer. This enzyme protects red blood cells and other cells from oxidative damage.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/500000.

1/500,000 on 500 ng of antigen.

IHC-P 1/1000 - 1/20000.

Dilution depending on tissue and detection method. Antigen retrieval has been used in characterisation but may not be necessary.

IHC-Fr 1/1000 - 1/20000.

Dilution depending on tissue and detection method.

WB 1/10000 - 1/100000. Predicted molecular weight: 22 kDa.

Dilution depending on detection method.

ELISA 1/2000 - 1/20000.

ターゲット情報

画像

  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/400) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit.
  • DAB staining of microglia with GPx1S in human brain tissue. Primary antibody (1/1000) secondary antibody donkey anti-sheep - biotin (1/3000) followed by Vector ABC kit. (Oil emersion).

参考文献

This product has been referenced in:
  • Kim IH  et al. Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region. Mol Med Rep 16:133-142 (2017). IHC ; Gerbil . Read more (PubMed: 28534982) »
  • Seeher S  et al. Secisbp2 is essential for embryonic development and enhances selenoprotein expression. Antioxid Redox Signal 21:835-49 (2014). WB ; Mouse . Read more (PubMed: 24274065) »

See all 5 Publications for this product

レビューと Q&A

Please check the online datasheets of all antibodies. You will find the required information.

Thanks!

Thank you very much for contacting us.


The possible rabbit specific antibodies are available as;


ICAM1: the rabbit protein sequence (http://www.uniprot.org/uniprot/Q75T02) does not show any homology with human protein (http:...

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Treatment
Selenium poor or adequate feeding
Gel Running Conditions
Reduced Denaturing (Gel 12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Aug 26 2009

Thank you for your enquiry and your patience. I apologize for the delay in obtaining the images, however, we now have some excellent images that we added to the online datasheet. This is a high titer antibody and when characterized on dot blots...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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