製品の概要
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製品名Anti-Glutaredoxin 5 antibody
Glutaredoxin 5 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Glutaredoxin 5
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
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免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (AVVQILRLHG VRDYAAYNVL DDPELRQGIK DYSNWPTIPQ VYLNGEFVGG) of Human Glutaredoxin 5 (NP_057501).
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ポジティブ･コントロール
- Transfected 293T cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab86411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1.
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関連疾患Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA) [MIM:205950]. A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus.
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配列類似性Belongs to the glutaredoxin family. Monothiol subfamily.
Contains 1 glutaredoxin domain. -
細胞内局在Mitochondrion.
- Information by UniProt
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参照データベース
- Entrez Gene: 51218 Human
- Entrez Gene: 73046 Mouse
- Entrez Gene: 362776 Rat
- Omim: 609588 Human
- SwissProt: Q86SX6 Human
- SwissProt: Q80Y14 Mouse
- Unigene: 532683 Human
- Unigene: 728210 Human
see all -
別名
- C14orf87 antibody
- Chromosome 14 open reading frame 87 antibody
- FLB4739 antibody
see all
画像
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Western blot - Anti-Glutaredoxin 5 antibody (ab86411)Anti-Glutaredoxin 5 antibody (ab86411) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Gel concentration 10-20%
プロトコール
参考文献
This product has been referenced in:
- Baker PR et al. Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5. Brain 137:366-79 (2014). WB ; Human . Read more (PubMed: 24334290) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"