Anti-Glutaredoxin 2 抗体 - C-terminal (ab85267)

製品の概要

  • 製品名Anti-Glutaredoxin 2 antibody - C-terminal
    Glutaredoxin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutaredoxin 2 - C-terminal
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide selected from the C terminal region of human Glutaredoxin 2 conjugated to KLH (NP_932066)

  • ポジティブ・コントロール
    • Mouse cerebellum tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • 組織特異性Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
  • 配列類似性Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • 細胞内局在Mitochondrion and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2) antibody
    • bA101E13.1 antibody
    • CGI133 antibody
    • Glrx2 antibody
    • GLRX2_HUMAN antibody
    • Glutaredoxin-2 antibody
    • Glutaredoxin-2, mitochondrial antibody
    • GRX2 antibody
    • mitochondrial antibody
    see all

Anti-Glutaredoxin 2 antibody - C-terminal 画像

  • Anti-Glutaredoxin 2 antibody - C-terminal (ab85267) at 1/50 dilution + mouse cerebellum tissue lysates at 35 µg

    Predicted band size : 18 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab85267 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glutaredoxin 2 antibody - C-terminal (ab85267) 使用論文

This product has been referenced in:
  • Wu H  et al. Glutaredoxin 2 (Grx2) gene deletion induces early onset of age-dependent cataracts in mice. J Biol Chem 289:36125-39 (2014). WB ; Mouse . Read more (PubMed: 25362663) »
  • Mailloux RJ  et al. Glutathionylation state of uncoupling protein-2 and the control of glucose-stimulated insulin secretion. J Biol Chem 287:39673-85 (2012). WB . Read more (PubMed: 23035124) »

See all 3 Publications for this product

Product Wall

Thank you for your response.

Unfortunately, the two attached images have not gone through successfully so I am unable to open them. It is reassuring that Gelsolin and Beta-actin antibodies provide clear signal but they are not positive contro...

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Thank you very much for getting back to me and for providing some further important details on the experiments. Your co-operation and effort is very much appreciated.

I can confirm that the expected band for the Glutaredoxin 2 is around 25 kD...

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Thank you for your enquiry regarding ab85267 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

Though you have kindly provided some det...

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Thank you for sending me your data and details of your protocol.  As we discussed, it is probable that the lowest band is the Glutaredoxin 2 protein, but I have no information as to why the band would appear in one sample and not the other.  You may be...

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