Anti-Glutaredoxin 1 抗体 (ab55059)

製品の概要

  • 製品名Anti-Glutaredoxin 1 antibody
    Glutaredoxin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Glutaredoxin 1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-107 of Human Glutaredoxin 1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
  • 配列類似性Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GLRX antibody
    • Glrx1 antibody
    • GLRX1_HUMAN antibody
    • Glutaredoxin antibody
    • Glutaredoxin-1 antibody
    • Glutaredoxin1 antibody
    • Grx 1 antibody
    • Grx antibody
    • Grx1 antibody
    • MGC117407 antibody
    • Thioltransferase 1 antibody
    • Thioltransferase antibody
    • Thioltransferase-1 antibody
    • Thioltransferase1 antibody
    • TTase antibody
    • TTase 1 antibody
    • TTase-1 antibody
    • TTase1 antibody
    see all

Anti-Glutaredoxin 1 antibody 画像

  • Glutaredoxin 1 antibody (ab55059) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 12 kDa
    Glutaredoxin 1 antibody (ab55059) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • Flow Cytometry analysis of Glutaredoxin 1 using ab55059.
    Human monocytes were fixed in paraformaldehyde and permeabilized. ab55059 was used at a 1/250 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) polyclonal, used at a 1/500 dilution.

    See Abreview

  • ab55059 staining Glutaredoxin 1 in human monocytes by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed, permeabilized, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab55059 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse IgG (H+L), used at a 1/1000 dilution.

    See Abreview

Anti-Glutaredoxin 1 antibody (ab55059) 使用論文

ab55059 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (RAW 264.7)
Specification RAW 264.7
Treatment Not Treated, Treated with 3.125,12.5 and 50 ug/mL CoO 60nm particles and 250 uM Diamide
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vamsi Kodali

Verified customer

投稿 Nov 27 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Monocytes)
Specification Monocytes
Fixative BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Feb 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Monocytes from Apheresis)
Specification Monocytes from Apheresis
Fixation BD Cytofix/Cytoperm 20 min
Permeabilization Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"