Anti-Glutamine Synthetase 抗体 (ab93439)
Key features and details
- Rabbit polyclonal to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Glutamine Synthetase antibody
Glutamine Synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Glutamine Synthetase -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Chicken, Cow, Dog, Human, Pig, Zebrafish, Cynomolgus monkey, Chinese hamster -
免疫原
Synthetic peptide corresponding to Mouse Glutamine Synthetase aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab103264) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93439の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). |
ターゲット情報
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機能
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
関連疾患
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
配列類似性
Belongs to the glutamine synthetase family. -
発生段階
Expressed during early fetal stages. -
細胞内局在
Cytoplasm. Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 396489 Chicken
- Entrez Gene: 281199 Cow
- Entrez Gene: 2752 Human
- Entrez Gene: 14645 Mouse
- Entrez Gene: 396944 Pig
- Entrez Gene: 24957 Rat
- Omim: 138290 Human
- SwissProt: P16580 Chicken
see all -
別名
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
画像
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All lanes : Anti-Glutamine Synthetase antibody (ab93439) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Cerebellum (Mouse) Tissue Lysate
Lane 4 : Cerebellum (Rat) Tissue Lysate
Lane 5 : Kidney (Mouse) Tissue Lysate
Lane 6 : Kidney (Rat) Tissue Lysate
Lane 7 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab93439 は 4 報の論文で使用されています。
- Mitra S et al. Dual regulation of lin28a by Myc is necessary during zebrafish retina regeneration. J Cell Biol 218:489-507 (2019). PubMed: 30606747
- Mitra S et al. Histone Deacetylase-Mediated Müller Glia Reprogramming through Her4.1-Lin28a Axis Is Essential for Retina Regeneration in Zebrafish. iScience 7:68-84 (2018). PubMed: 30267687
- Morales I et al. Striatal astrocytes engulf dopaminergic debris in Parkinson's disease: A study in an animal model. PLoS One 12:e0185989 (2017). PubMed: 29028815
- Morales I et al. The astrocytic response to the dopaminergic denervation of the striatum. J Neurochem 139:81-95 (2016). PubMed: 27230040