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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Glutamine Synthetase.
(Peptide available as ab73592.)
Our Abpromise guarantee covers the use of ab73593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Mouse Glutamine Synthetase peptide (ab73592).|
Immunohistochemistical detection of Glutamine Synthetase on Formaldehyde-fixed paraffin-embedded rat brain sections using Glutamine Synthetase antibody (ab73593). Antigen retrieval step: Heat mediated - Buffer/Enzyme Used: Citric acid pH6 Permeabilization. Blocking: 1% BSA for 10 mins @ rt°C. Primary Antibody Dilution 1/1000. Incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG Conjugated to biotin (1/200). In the submitted image of rat cerebellum, two very good examples are observed of the bulbous endfeet of the primary Bergmann glia primary processes connecting with the inner Pial membrane.