製品の概要

  • 製品名Anti-Glutamine Synthetase antibody
    Glutamine Synthetase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Glutamine Synthetase
  • アプリケーション適用あり: WB, IP, ELISA, Dot blotmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Glutamine Synthetase [Microbial]

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • 精製度Whole antiserum
  • 特記事項(精製)This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamine Synthetase [Microbial].
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
ELISA
Dot blot
  • 追加情報immunoblotting (western or dot blot), ELISA (1:10,000 to 1:40,000), immunoprecipitation and most immunological methods requiring high titer and specificity.
  • ターゲット情報

    • 機能This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
    • 関連疾患Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
    • 配列類似性Belongs to the glutamine synthetase family.
    • 発生段階Expressed during early fetal stages.
    • 細胞内局在Cytoplasm. Mitochondrion.
    • Information by UniProt
    • 別名
      • cell proliferation-inducing protein 59 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    Anti-Glutamine Synthetase antibody (ab6585) 使用論文

    This product has been referenced in:
    • Ivanovska N & Dimitrova P Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine. Arthritis Res Ther 13:R44 (2011). IHC-P ; Mouse . Read more (PubMed: 21410959) »
    • Yuan J  et al. Metabolomics-driven quantitative analysis of ammonia assimilation in E. coli. Mol Syst Biol 5:302 (2009). WB ; Escherichia coli . Read more (PubMed: 19690571) »

    See all 2 Publications for this product

    Product Wall

    They have informed me that for both ab6585 and ab34525, they do not have any data on possible cross-reactivity with Streptomyces lincolnensis. The actual immunogen is not sequenced but a reference sequence for comparison is shown at: http://www.un...

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    I suggest that you conduct your search as normal through the Abcam Website. As you cannot find a product within the Abcam catalog that meets your needs, click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search ...

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    I've checked our previous enquiries for this antibody and it seems that there are lots of questions regarding the nature of the immunogen, but I'm afraid that I do not know from which microbe the GS was derived. The antibody has not specifically been t...

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    We have contacted the manufacturer of the enzyme used, and they can't tell us which isoform of Gln synthetase was used. Apparently, they measure activity in a set assay and if the enzyme (or enzymes) perform properly, they don't worry about which isofo...

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    This antibody was raised against a microbial glutamine synthetase and has not been specifically tested for cross-reactivity with the rat enzyme. Neither has it been used previously in immunocytochemistry, however it is thought likely that it would work...

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    This antibody has been raised against microbial glutamine synthetase and does not react with the mouse protein.

    This antibody has not been specifically tested in IHC and IF. Unfortunately, we do not know of any references where this antibody has been used.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"