製品の概要

  • 製品名Anti-Glutamine Synthetase antibody
    Glutamine Synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutamine Synthetase
  • 特異性ab49873 recognizes glutamine synthetase (GS) (45 kDA).
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Monkey
    交差が予測される動物種: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    RTCLLNETGDEPFQYKN

    , corresponding to C terminal amino acids 357-373 of Mouse Glutamine Synthetase

  • ポジティブ・コントロール
    • Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Ion Exchange Chromatography
  • 特記事項(精製)Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/10000 - 1/20000.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.

ターゲット情報

  • 機能This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • 関連疾患Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • 配列類似性Belongs to the glutamine synthetase family.
  • 発生段階Expressed during early fetal stages.
  • 細胞内局在Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Anti-Glutamine Synthetase antibody 画像

  • ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.

    See Abreview

  • Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
    .

    See Abreview

Anti-Glutamine Synthetase antibody (ab49873) 使用論文

This product has been referenced in:
  • Eakins R  et al. Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome. Sci Rep 5:16423 (2015). Read more (PubMed: 26607827) »
  • Zhang S  et al. TALEN-mediated somatic mutagenesis in murine models of cancer. Cancer Res 74:5311-21 (2014). Mouse . Read more (PubMed: 25070752) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Jugular nodose ganglion complex (JNC))
Antigen retrieval step None
Permeabilization No
Specification Jugular nodose ganglion complex (JNC)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative Zamboni
Username

Mr. Duc Dung Le

Verified customer

投稿 Apr 20 2015

Application IHC - Wholemount
Sample Mouse Tissue (Retina)
Specification Retina
Username

Abcam user community

Verified customer

投稿 Feb 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 12 2013

Thank you for your call today and for letting us know about this issue.
I've created a discount code that can be used to receive 20% off a vial of ab49873. Please include the code below with your order to redeem this offer:
Discount Code: ***...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Telefon diskutiert, ist unseres Wissens nach wurde der ab49873 bisher noch nicht inauf humanem Gewebegetestet. Falls Sie dies selbst testen möchten...

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That's no problem. I am sorry for the confusion, I should have made it clear to you that they would be arriving in the same box.

If I can help in any other way please do let me know. Otherwise, I wish you all the best with your research.

That is no problem. If you have any problems in the future, please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Thank you for getting back to me.

I am sorry for the frustration that this situation has caused you. I will arrange for ab91073 to be sent out to you as discussed. I can also arrange for ab60709 to be sent as a replacement for ab49873 which y...

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Thank you for getting back to me.

I am sorry that the reply to your fax was sent to your supervisor by one of my colleagues, Keith. This was due to the way our assigning system works. The protocol tips that I gave and those of Keith are not t...

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Thank you for providing me with this extra information. It has allowed me to more fully understand the problems you have been experiencing with ab49873 and ab93434 previously.

Having looked at the protocol I have a few further questions:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"