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Recombinant full length protein purified from E.coli (Human).
Our Abpromise guarantee covers the use of ab16802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 42 kDa.|
Left panel shows scanned image of an amine coated plate seeded with a titration of HeLa cells and stained with different dilutions of ab16082 using In Cell ELISA support pack ab111542 and protocol. Right panel shows bar graph of normalized signal intensity in 3 different cells lines after staining with ab160802 at 1/2500 dilution. Average intra-assay coefficient of variation was calculated at 5.6% and Z score at 0.77.