Anti-Glutamate receptor ionotropic, NMDA 2D 抗体 (ab35448)

製品の概要

  • 製品名
    Anti-Glutamate receptor ionotropic, NMDA 2D antibody
    Glutamate receptor ionotropic, NMDA 2D 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutamate receptor ionotropic, NMDA 2D
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glutamate receptor ionotropic, NMDA 2D aa 1300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O15399
    (Peptide available as ab35447)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab35448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).Can be blocked with Rat Glutamate receptor ionotropic or NMDA 2D peptide (ab35447).

ターゲット情報

  • 機能
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • 配列類似性
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.
  • 細胞内局在
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EB11 antibody
    • estrogen receptor binding CpG island antibody
    • GluN2D antibody
    • Glutamate [NMDA] receptor subunit epsilon 4 antibody
    • Glutamate [NMDA] receptor subunit epsilon-4 antibody
    • Glutamate receptor ionotropic antibody
    • glutamate receptor ionotropic NMDA 2D antibody
    • Glutamate receptor, ionotropic, N-methyl D-aspartate 2D antibody
    • Grin2d antibody
    • N methyl D aspartate receptor subtype 2D antibody
    • N methyl d aspartate receptor subunit 2D antibody
    • N-methyl D-aspartate receptor subtype 2D antibody
    • NMDA 2D antibody
    • NMDAR2D antibody
    • NMDE4 antibody
    • NMDE4_HUMAN antibody
    • NR2D antibody
    see all

画像

  • All lanes : Anti-Glutamate receptor ionotropic, NMDA 2D antibody (ab35448) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
    Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
    Lane 4 : Brain (Rat) Tissue Lysate, 7 days old

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 143 kDa
    Observed band size : 143 kDa
    Additional bands at : 125 kDa,45 kDa,60 kDa,~200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

プロトコール

参考文献

This product has been referenced in:
  • Zhou X  et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet 19:3797-805 (2010). WB ; Mouse . Read more (PubMed: 20634195) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (e12 PNS and adult cerebellum)
Specification
e12 PNS and adult cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid ph6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Jul 19 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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