Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) 抗体 [EPR2148] (ab76321)

製品の概要

  • 製品名Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148]
    Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
  • 特異性ab76321 only detects Glutamate Receptor 1 (AMPA subtype) phosphorylated on Serine 845.
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).

  • ポジティブ・コントロール
    • WB: Human fetal, rat and mouse brain tissue lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 102 kDa.

For unpurified use at 1/1000 - 1/10000.

  • 追加情報Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • 組織特異性Widely expressed in brain.
    • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • 翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] 画像

    • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1000 cells (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate, untreated
      Lane 2 : Mouse brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (unpurified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) 使用論文

    ab76321 has not yet been referenced specifically in any publications.

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