Anti-Glutamate Receptor 1 (AMPA subtype) 抗体 (ab31232)

製品の概要

  • 製品名Anti-Glutamate Receptor 1 (AMPA subtype) antibody
    Glutamate Receptor 1 (AMPA subtype) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype)
  • アプリケーション適用あり: WB, IHC-FoFr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Marmoset (common)
    交差が予測される動物種: Monkey
  • 免疫原

    Synthetic peptide derived from within residues 850 to the C-terminus of Human Glutamate Receptor 1 (AMPA subtype).

    (Peptide available as ab28424.)

  • ポジティブ・コントロール
    • Mouse Brain Tissue Lysate;

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.Can be blocked with Human Glutamate Receptor 1 (AMPA subtype) peptide (ab28424).
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • 組織特異性Widely expressed in brain.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • 翻訳後修飾Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Anti-Glutamate Receptor 1 (AMPA subtype) antibody 画像

  • Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype)  with ab31232 on formaldehyde-fixed paraffin-embedded rat cerebellum sections. Antigen retrieval step: heat mediated  in citric acid pH6. Blocking step: 1% BSA  for 10 mins @ rt°C.  Primary antibody was incubated at 1/200 for 2 hours @ room temp. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows localisation of this protein apparently exclusive to the molecular layer of the cerebellum upper image (low power). In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted.

    See Abreview

  • Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) at 1 µg/ml + Mouse Brain Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab31232 staining Glutamate Receptor 1 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. 

    See Abreview

  • Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) with ab31232 on  formaldehyde-fixed paraffin-embedded mouse brain sections. Antigen retrieval step: heat mediated in Citric acid pH6 buffer.Blocking step: 1%BSA for 10 mins @ rt°C. Primary antibody ab31232 incubated at 1/100 for 2 hours. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows immunostaining in cell bodies and processes of a subset of neurones in the mouse cortex (upper image) and also in the dendritic fields of the molecular layer of the mouse cerebellum (lower image). Purkinje cells of the cerebellum are negative but their primary processes are clearly delineated by a punctate membrane-region positivity. What appears to be small cells in close contact with the Purkinje cell bodies are strongly positive (perhaps these are basket cells?).
  • IHC image of Glutamate Receptor 1 (AMPA subtype) staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31232, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) 使用論文

This product has been referenced in:

See all 23 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (adult spinal cord)
Permeabilization Yes - Triton X-100, 0.5%
Specification adult spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 22 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
Username

Dr. Sarah Franklin

Verified customer

投稿 Aug 14 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification hippocampi total membrane fraction
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 02 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 10 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Cell lysate - whole cell (Mouse and Rat Hippocampus)
Specification Mouse and Rat Hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 22 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset Cerebellum)
Specification Marmoset Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (cerebellum)
Specification cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Feb 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Feb 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"