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Glutamate Assay Kit (ab83389) provides a sensitive detection method of the glutamate in a variety of samples. This kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins. The glutamate Enzyme Mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.
EDTA plasma may not be used with the ab83389 Glutamate Assay Kit. EDTA can interfere with the assay.
Visit our FAQs page for tips and troubleshooting.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
|Glutamate Assay Buffer||WM||1 x 25ml|
|Glutamate Developer (Lyophilised)||Red||1 vial|
|Glutamate Enzyme Mix (Lyophilised)||Green||1 vial|
|Glutamate Standard (0.1M)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab83389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|