製品の概要

  • 製品名Glutamate Assay Kit
    Glutamate キット 製品一覧
  • テスト
    100 x 1 assay
  • サンプルの種類
    Urine, Serum, Plasma, Other biological fluids, Cell Lysate
  • アッセイタイプQuantitative
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Glutamate Assay Kit (ab83389) provides a sensitive detection method of the glutamate in a variety of samples. This kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins. The glutamate Enzyme Mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.

     

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

アプリケーション

Our Abpromise guarantee covers the use of ab83389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Glutamate Assay Kit 画像

  • Example of Glutamate standard curve obtained using ab83389

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Glutamate Assay Kit (ab83389) 使用論文

This product has been referenced in:
  • Evonuk KS  et al. Inhibition of System Xc(-) Transporter Attenuates Autoimmune Inflammatory Demyelination. J Immunol 195:450-63 (2015). Read more (PubMed: 26071560) »
  • Sloniecka M  et al. Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ. PLoS One 10:e0134157 (2015). Functional Studies . Read more (PubMed: 26214847) »

See all 4 Publications for this product

Product Wall

Abreviews
I used lysates of mouse hippocampus. Since the "enzyme" is present in fresh lysates, deproteinization is required
http://www.abcam.com/protocols/deproteinization-protocol
Don't forget to leave aside sample for Bradford normalization before deproteinization
Username

Dr. Tamar Licht

Verified customer

投稿 Mar 08 2016



I have contacted the lab and they have confirmed that we can add the Glutamate assay buffer to the catalogue to purchase separately to dilute your samples.

However, the lab have also said that you can simply dilute yoursamples in dei...

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Préparation de plasma:

Prélever le sang dans un tube de « sodium heparin » (par exemple BD Vacutainer, Cat No:. 8001302 ou 16852)
Centrifuger pendant 10 minutes à 3000 rpm
Faire des aliquots e...

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Unfortunately neither of these kits will be able to detect glutamic acid in peptides/proteins. ab83389 only measures free glutamate levels not glutamic acid and ab138883 is based on enzymatic reactions so it is most likely that it will not react with t...

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Non normalement les ions NH3 ne devraient pas interférer le dosage avec ce kit.



The Glutamate Assay Kit (ab83389) can be used to asses the level of glutamate in a range of different biological samples. Unfortunately, the dilution range required will need to be assessed for the different samples you are hoping to use the ...

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I've checked with the laboratory, and this kit is very specific for glutamate only. Glutamine is not going to cross react and produce false signals. So, no optimization is required and the glutamine media will be fine.

J’ai eu une réponse du laboratoire concernant la préparation de vos échantillons. Les composants de votre tampon ne devraient pas causer d’interférence avec ce kit. Cependant, le laboratoire recommande de diluer ...

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Thank you for contacting us.

After doing some research on this subject it seems that astrocytes release glutamate in a Ca2+ dependent manner. It is a very well-known function/property of astrocytes involving transmitter uptake and release. Read More

Thank you for contacting us.
Though these kits has not been tested with rat samples however based on the material used in these kits these will compatible with all type of mammalian cells.
I can confirm that these kits will be suitable for Ra...

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1-10 of 36 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"