製品の概要

  • 製品名Anti-GLUT12 antibody
    GLUT12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GLUT12
  • 特異性This antibody has been fully characterized for cross reactivity within the Glut family and with other cellular proteins.
  • アプリケーション適用あり: ICC/IF, WB, ELISA, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C-terminus of Rat GLUT12. (Peptide available as ab82700.)

  • ポジティブ・コントロール
    • Hippocampal plasma membranes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500. Predicted molecular weight: 67 kDa.
ELISA 1/4500.
Dot blot 1/4500.

ターゲット情報

  • 機能Facilitative glucose transporter.
  • 組織特異性Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily perinuclear region in the absence of insulin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glucose transporter type 12 antibody
    • GLUT 12 antibody
    • GLUT-12 antibody
    • GLUT12 antibody
    • GLUT8 antibody
    • GTR12_HUMAN antibody
    • SLC2A12 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 12 antibody
    • Solute carrier family 2 facilitated glucose transporter member 12 antibody
    • Solute carrier family 2 member 12 antibody
    • Solute carrier family 2, facilitated glucose transporter member 12 antibody
    see all

Anti-GLUT12 antibody 画像

  • ICC/IF image of ab75441 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75441, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GLUT12 antibody (ab75441) 使用論文

This product has been referenced in:
  • de Laat MA  et al. Hyperinsulinemia Down-Regulates TLR4 Expression in the Mammalian Heart. Front Endocrinol (Lausanne) 5:120 (2014). WB ; Rat . Read more (PubMed: 25101057) »
  • Pyla R  et al. Expression of conventional and novel glucose transporters, GLUT1, -9, -10, and -12, in vascular smooth muscle cells. Am J Physiol Cell Physiol 304:C574-89 (2013). Read more (PubMed: 23302780) »

See all 4 Publications for this product

Product Wall

Thank you very much for your inquiry.

I can confirm that we do not have any antibodies against GLUT11.

But I am happy to let you know that we have an anti GLUT7 antibody that is tested and guaranteed for IHC-P. It is named after SLC...

Read More
Application Western blot
Sample Horse Tissue lysate - whole (skeletal muscle)
Loading amount 25 µg
Specification skeletal muscle
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"