製品の概要

  • 製品名Anti-GLUT12 antibody
    GLUT12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GLUT12
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human GLUT12.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human liver membrane and Human liver tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能Facilitative glucose transporter.
  • 組織特異性Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily perinuclear region in the absence of insulin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glucose transporter type 12 antibody
    • GLUT 12 antibody
    • GLUT-12 antibody
    • GLUT12 antibody
    • GLUT8 antibody
    • GTR12_HUMAN antibody
    • SLC2A12 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 12 antibody
    • Solute carrier family 2 facilitated glucose transporter member 12 antibody
    • Solute carrier family 2 member 12 antibody
    • Solute carrier family 2, facilitated glucose transporter member 12 antibody
    see all

Anti-GLUT12 antibody 画像

  • All lanes : Anti-GLUT12 antibody (ab100993) at 1 µg/ml

    Lane 1 : Human liver tissue lysate (cirrhosis) - membrane extract (ab29881)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab100993 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab100993 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GLUT12 antibody (ab100993) 使用論文

ab100993 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your inquiry.

I can confirm that we do not have any antibodies against GLUT11.

But I am happy to let you know that we have an anti GLUT7 antibody that is tested and guaranteed for IHC-P. It is named after SLC...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"