製品の概要

  • 製品名Anti-GLUD1 antibody
    GLUD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GLUD1
  • 特異性Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Saccharomyces cerevisiae, Chimpanzee, African Green Monkey
  • 免疫原

    Synthetic peptide corresponding to Human GLUD1 aa 72-121 (N terminal).
    Database link: P00367

  • ポジティブ・コントロール
    • HT1080 cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human liver cancer.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
  • 関連疾患Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
  • 配列類似性Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI118167 antibody
    • DHE3_HUMAN antibody
    • EC 1.4.1.3 antibody
    • GDH 1 antibody
    • GDH antibody
    • Gdh-X antibody
    • GDH1 antibody
    • GLUD antibody
    • Glud1 antibody
    • Glud1a antibody
    • Glud1b antibody
    • Gludl antibody
    • Glutamate dehydrogenase (NAD(P)+) antibody
    • Glutamate dehydrogenase 1 antibody
    • Glutamate dehydrogenase 1 mitochondrial antibody
    • Glutamate dehydrogenase 1a antibody
    • Glutamate dehydrogenase 1b antibody
    • Memory-related gene 2 protein antibody
    • MGC127177 antibody
    • MGC132003 antibody
    • MGC80801 antibody
    • MGC93608 antibody
    • mitochondrial antibody
    • MRG-2 antibody
    see all

Anti-GLUD1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancrease tissue labelling GLUD1 with ab89967.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling GLUD1 with ab89967.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney (cortex) tissue labelling GLUD1 with ab89967.

  • Anti-GLUD1 antibody (ab89967) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
  • IHC image of GLUD1 staining in Human liver cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89967, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GLUD1 antibody (ab89967) 使用論文

This product has been referenced in:
  • Broniarek I  et al. The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells. Pflugers Arch 468:1541-54 (2016). WB ; Human . Read more (PubMed: 27417103) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"