The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/6000 - 1/24000.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
機能May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
関連疾患Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
配列類似性Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Human Pancreas (formalin fixed, paraffin embedded tissue) stained with ab116714 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Anti-GLUD1 antibody (ab116714) 使用論文
has not yet been referenced specifically in any publications.