Anti-Glucosidase 2 subunit beta 抗体 (ab125869)

製品の概要

  • 製品名
    Anti-Glucosidase 2 subunit beta antibody
    Glucosidase 2 subunit beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucosidase 2 subunit beta
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 237-460 of Human Glucosidase 2 subunit beta (P14314).

  • ポジティブ・コントロール
    • A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab125869 is purified by antigen-affinity chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/20000. Predicted molecular weight: 59 kDa.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Regulatory subunit of glucosidase II.
  • パスウェイ
    Glycan metabolism; N-glycan metabolism.
  • 関連疾患
    Defects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.
  • 配列類似性
    Contains 2 EF-hand domains.
    Contains 1 PRKCSH domain.
  • 細胞内局在
    Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 80K-H protein antibody
    • AGE-binding receptor 2 antibody
    • AGE-R2 antibody
    • G19P1 antibody
    • GLU2B_HUMAN antibody
    • Glucosidase 2 subunit beta antibody
    • Glucosidase II beta subunit antibody
    • Glucosidase II subunit beta antibody
    • Hepatocystin antibody
    • PCLD antibody
    • PKCSH antibody
    • PLD1 antibody
    • PRKCSH antibody
    • Protein kinase C substrate 60.1 kDa protein heavy chain antibody
    • Protein kinase C substrate 80 Kda protein antibody
    • Protein kinase C substrate 80K-H antibody
    • Protein kinase C substrate, 80 Kda protein antibody
    see all

画像

  • All lanes : Anti-Glucosidase 2 subunit beta antibody (ab125869) at 1/10000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 59 kDa
    7.5% SDS PAGE
  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Glucosidase 2 subunit beta (green) using ab125869 at 1/500 dilution in ICC/IF. 

    Blue: Hoechst 33342 staining.

参考文献

ab125869 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP)
Specification
LNCaP
Fixative
Formaldehyde
Permeabilization
No
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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