製品の概要

  • 製品名
    Anti-Glucose Transporter GLUT4 antibody
    Glucose Transporter GLUT4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose Transporter GLUT4
  • 特異性
    GLUT4.
  • アプリケーション
    適用あり: ICC/IF, WB, IP, Immunomicroscopy, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Pig, Armenian hamster
  • 免疫原

    Synthetic peptide corresponding to Rat Glucose Transporter GLUT4 aa 1-15 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115831)

  • ポジティブ・コントロール
    • IHC-P: FFPE human heart tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000.
WB 1/2000 - 1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 54.8 kDa).Can be blocked with Rat Glucose Transporter GLUT4 peptide (ab115831).

The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.

It is recomended to use 3% milk block.

IP Use at an assay dependent concentration. PubMed: 18234908
Immunomicroscopy Use at an assay dependent concentration. Can also can be used for Immunogold EM (purified IgG). See references for specific protocols.
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Insulin-regulated facilitative glucose transporter.
  • 組織特異性
    Skeletal and cardiac muscles; brown and white fat.
  • 関連疾患
    Diabetes mellitus, non-insulin-dependent
  • 配列類似性
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾
    Sumoylated.
  • 細胞内局在
    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • 参照データベース
  • 別名
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Anti-Glucose Transporter GLUT4 antibody 画像

  • Anti-Glucose Transporter GLUT4 antibody (ab654) at 1/1000 dilution (Diluted with TBST and Incubated for 16 hours at 4°C) + Mouse Heart at 30 µg with Milk, 1 hour at 25°C at 3 %

    Secondary
    Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 54.8 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds

    Image courtesy of an anonymous Abreview

    See Abreview

  • IHC image of Glucose Transporter GLUT4 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab654, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab654 staining GLUT4 in human mesenchymal stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Horse serum) for 1 hour at 22°C. A FITC-conjugated rabbit polyclonal was used as the secondary antibody (1/100).

    See Abreview

  • ab654 at a 1/300 dilution, staining human GLUT4 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab654 staining GLUT4 in human endometriosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 3% BSA in TBS) for 16 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab654 at 1/500 dilution staining GLUT4 in mouse muscle by immunohistochemistry (frozen sections). Sections were methanol fixed prior to blocking in 5% goat serum for 1 hour at 25°C and then incubated with ab654 for 1 hour at 25°C. A goat polyclonal to rabbit IgG, diluted 1/500, was used as the secondary antibody.

    See Abreview

Anti-Glucose Transporter GLUT4 antibody (ab654) 使用論文

This product has been referenced in:
  • Lu H  et al. Transient hypoxia reprograms differentiating adipocytes for enhanced insulin sensitivity and triglyceride accumulation. Int J Obes (Lond) 40:121-8 (2016). Mouse . Read more (PubMed: 26219415) »
  • Zhou Y  et al. MicroRNA-29a induces insulin resistance by targeting PPARd in skeletal muscle cells. Int J Mol Med 37:931-8 (2016). WB ; Rat . Read more (PubMed: 26936652) »

See all 68 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placental Explant)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Placental Explant
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Jeremiah Gaudet

Verified customer

投稿 Aug 01 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle, and retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
30 µg
Specification
Mouse brain, heart, liver, muscle, and retina
Blocking step
Licor Odyssey Blocking BUffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 23 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (gel 4-15% SDS)
Loading amount
30 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 04 2015

I would recommend looking at the human protein atlas which shows in which tissues the protein is expressed:

http://www.proteinatlas.org/ENSG00000181856/tissue

From this, it would seem that the following tissues could be good negative ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Human Tissue sections (Endometriosis)
Specification
Endometriosis
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Brett Mckinnon

Verified customer

投稿 Jun 10 2013

Hello and thank you for sending your image. We do highly recommend boiling your samples with this antibody, and this may reduce background. However, this target is phosphorylated and glycosylated which is another reason you may see two strong bands. If...

Read More

Thank you for your new contact. My colleague is out of office today and she has asked me to look after her customers.

Currently, we do not have a different Western blot image available.The predicted molecular weight of GLUT4 is54.8 kDa, howev...

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mesenchymal stem cells)
Specification
Mesenchymal stem cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Steven Johnstone

Verified customer

投稿 Sep 11 2012

Thank you for contacting us.

All the images from WB assay in the datasheet belong to Abreviews from other users. I am afraid we do not have access to the complete images.

As new images or references are brought to our attention, the...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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