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Synthetic peptide from the C terminus of Glucose Transporter GLUT3.
Our Abpromise guarantee covers the use of ab41525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/8000. Detects a band of approximately 45-47 kDa.Can be blocked with Glucose Transporter GLUT3 peptide (ab105625). Detects a band of approximately 45-47 kDa using 10ug hippocampal plasma membranes as antigen. The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.|
|IP||Use at an assay dependent concentration. Use at an assay dependent dilution. 2 ul ab41525 will immunoprecipitate 80-85% GLUT3 from 250 ug rat solublized hippocampal membranes|
|Dot blot||1/25000. With solublized hippocampal plasma membranes.|
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 4 µg/ml.|