Anti-Glucose Transporter GLUT3 抗体 (ab15311)

製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Facilitative glucose transporter. Probably a neuronal glucose transporter.
  • 組織特異性
    Highly expressed in brain. Expressed in many tissues.
  • 配列類似性
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • brain antibody
    • FLJ90380 antibody
    • Glucose transporter type 3 antibody
    • Glucose transporter type 3 brain antibody
    • GLUT 3 antibody
    • GLUT-3 antibody
    • GLUT3 antibody
    • GTR3_HUMAN antibody
    • Slc2a3 antibody
    • Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody
    • Solute carrier family 2, facilitated glucose transporter member 3 antibody
    see all

Anti-Glucose Transporter GLUT3 antibody 画像

  • ab15311 staining Glucose Transporter GLUT3 in human placenta by Immunohistochemistry (FFPE-sections).
  • All lanes : Anti-Glucose Transporter GLUT3 antibody (ab15311) at 1/400 dilution

    Lane 1 : Lysate prepared from U87-MG cells
    Lane 2 : Lysate prepared from human brain
    Lane 3 : Lysate prepared from human brain
    Lane 4 : Lysate prepared from lymphoblastoid cell line

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat anti-rabbit IgG-HRP at 1/7500 dilution

    Predicted band size : 60 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa (possible glycosylated form).

    Image courtesy of Georg Ziegler by Abreview.

    See Abreview

Anti-Glucose Transporter GLUT3 antibody (ab15311) 使用論文

This product has been referenced in:
  • Kuang R  et al. GLUT3 upregulation promotes metabolic reprogramming associated with antiangiogenic therapy resistance. JCI Insight 2:e88815 (2017). WB ; Human . Read more (PubMed: 28138554) »
  • Vittori A  et al. Copy-number variation of the neuronal glucose transporter gene SLC2A3 and age of onset in Huntington's disease. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24452335) »

See all 14 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
Permeabilization
No
Specification
Testis
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Human Tissue sections (Endometriosis)
Specification
Endometriosis
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Brett Mckinnon

Verified customer

投稿 Jun 10 2013

Thank you for your reply and for these additional details.

I can see no apparent reason why this product would be giving you this pattern with your rat cortex samples independent of age or treatment. It is especially strange to me that it is...

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Thank you for contacting us.

Your issue is very interesting as some animals because of the variablity between samples independent of age or treatment. Would you be able to provide your WB protocols and sample treatments? It may be an issue...

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Vielen Dank für Ihre Anfrage und für Ihre Geduld.

Leider haben wir für die Antikörper ab15311 und ab53095 kein Blockpeptid, und können auch keines herstellen oder beschaffen, aber wir haben eines für den ab41525. Dieses hat die Katalognummer...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements.

To check the status of the order please contact our Customer Service team ...

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Vielen Dank für Ihre Anfrage. Es freut mich bestätigen zu können, dass beide Antikörper keine Lot-spezifische Konzentration aufweisen. ab14994 hat eine Konzentration von 1mg/ml und ab15311 hat eine Konzentration von 0.2mg/ml. Bitte folgen Sie diesen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U87-MG, human brain, lymphoblastoid cell-line)
Loading amount
15 µg
Specification
U87-MG, human brain, lymphoblastoid cell-line
Gel Running Conditions
Reduced Denaturing (4-12 % Bis-Tris Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Georg Ziegler

Verified customer

投稿 Jan 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount
30 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (10% gel + glycerol)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Kristina Terrell

Verified customer

投稿 Aug 20 2010

Thank you for your enquiry. I was able to obtain the immunogen sequence. This has been updated on the datasheet: SIEPAKETTTNV aa 485-496 of human Unfortunately, it appears there is no significant homology between the chicken sequence and the imm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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