Anti-Glucose Transporter GLUT2 抗体 (ab54460)

製品の概要

  • 製品名Anti-Glucose Transporter GLUT2 antibody
    Glucose Transporter GLUT2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose Transporter GLUT2
  • 特異性This antibody recognizes the ~53-61 kDa GLUT2 protein. It does not recognize other GLUT isoforms.
  • アプリケーション適用あり: WB, Indirect ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide conjugated to KLH corresponding to amino acids in the cytoplasmic domain of rat Glucose Transporter GLUT2.

  • 特記事項Following initial thaw, aliquot and freeze (-20°C).

アプリケーション

Our Abpromise guarantee covers the use of ab54460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 57 kDa.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
  • 組織特異性Liver, insulin-producing beta cell, small intestine and kidney.
  • 関連疾患Fanconi-Bickel syndrome
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • liver antibody
    • Glucose Transporter 2 antibody
    • Glucose Transporter GLUT2 antibody
    • Glucose transporter type 2 antibody
    • Glucose transporter type 2 liver antibody
    • Glucose transporter, liver/islet antibody
    • GLUT-2 antibody
    • GLUT2 antibody
    • GTR2_HUMAN antibody
    • GTT2 antibody
    • SLC2A2 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
    • Solute carrier family 2 facilitated glucose transporter member 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 2 antibody
    see all

Anti-Glucose Transporter GLUT2 antibody 画像

  • ICC/IF image of ab54460 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54460, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT2 antibody (ab54460) 使用論文

This product has been referenced in:
  • Pettinato G  et al. ROCK inhibitor is not required for embryoid body formation from singularized human embryonic stem cells. PLoS One 9:e100742 (2014). ICC/IF ; Human . Read more (PubMed: 25365581) »
  • Pettinato G  et al. Formation of well-defined embryoid bodies from dissociated human induced pluripotent stem cells using microfabricated cell-repellent microwell arrays. Sci Rep 4:7402 (2014). Read more (PubMed: 25492588) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MRC5 normal lung fibroblasts)
Specification MRC5 normal lung fibroblasts
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
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Dr. Dimitra Kalamida

Verified customer

投稿 Feb 27 2015

The ab8297 and ab85715 has been added to the order 1075844.

Thank you very muchfor your cooperation in this case.

Could you let me know which antibody you are interested in to get we have following antibodies in catalogue;

ab85715 and ab95256 are tested in ICC/IF on human cells

ab115199 and ab8297 are also tested in ICC/IF on human cells.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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Vielen Dank für Ihre Anfrage.

Leider haben wir keinen Antikörper, der allen genannten Anforderungen entspricht.

Wir führen einen Maus-monoklonalen GLUT2-Antikörper, der bislang nur für humane Proben getestet wurde (ab85715). Das I...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab54460Glucose Transporter GLUT2 antibodyhas never been tested in immunohistochemistry on tissue sections (IHC-P paraffin embedded or IHC-Fr Frozen). All testedap...

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Thank you for your message. I can confirm that the immunogen is as follows on the datasheet: A synthetic peptide conjugated to KLH corresponding to amino acids in the cytoplasmic domain of rat Glucose Transporter GLUT2. This is therefore raised a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"