Anti-Glucose Transporter GLUT10 抗体 (ab110528)

製品の概要

  • 製品名Anti-Glucose Transporter GLUT10 antibody
    Glucose Transporter GLUT10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose Transporter GLUT10
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Glucose Transporter GLUT10.

  • ポジティブ・コントロール
    • This antibody gave positive in Human Pancreas tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Facilitative glucose transporter.
  • 組織特異性Widely expressed; highest levels in liver and pancreas.
  • 関連疾患Defects in SLC2A10 are the cause of arterial tortuosity syndrome (ATS) [MIM:208050]. ATS is an autosomal recessive disorder characterized by tortuosity and elongation of major arteries, often resulting in death at young age. Other typical features include aneurysms of large arteries and stenosis of the pulmonary artery, in association with facial features and several connective tissue manifestations such as soft skin and joint laxity. Histopathological findings include fragmentation of elastic fibers in the tunica media of large arteries.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 細胞内局在Endomembrane system. Cytoplasm > perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA450473 antibody
    • facilitated glucose transporter member 10 antibody
    • Glucose transporter type 10 antibody
    • glut 10 antibody
    • GLUT-10 antibody
    • GTR10_HUMAN antibody
    • MGC126706 antibody
    • slc2a10 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 10 antibody
    see all

Anti-Glucose Transporter GLUT10 antibody 画像

  • IHC image of Glucose Transporter GLUT10 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110528, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Glucose Transporter GLUT10 antibody (ab110528) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-Glucose Transporter GLUT10 antibody (ab110528) 使用論文

ab110528 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"