Anti-Glucose Transporter GLUT1 抗体 [EPR3915] (ab115730)

製品の概要

  • 製品名Anti-Glucose Transporter GLUT1 antibody [EPR3915]
    Glucose Transporter GLUT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glucose Transporter GLUT1 aa 450 to the C-terminus.
    (Peptide available as ab202335)

  • ポジティブ・コントロール
    • Jurkat, HepG2, 3T3L1, Mouse brain, Human fetal brain and Human fetal liver lysates; Human cervical carcinoma and colonic adenocarcinoma tissues.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/500.
WB 1/100000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)

Flow Cyt 1/40.

For unpurified, use 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • 組織特異性Expressed at variable levels in many human tissues.
  • 関連疾患Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Anti-Glucose Transporter GLUT1 antibody [EPR3915] 画像

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/100000 dilution (purified)

    Lane 1 : NIH/3T3 whole cell lysate
    Lane 2 : PC-12 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry.

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/200000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : 3T3-L1 brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000000 dilution (purified)

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : HT-29 whole cell lysate
    Lane 4 : SW480 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 54 kDa
    Observed band size : 40-60 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Immunofluorescence staining of HepG2 cells with purified ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Overlay histogram showing HeLa cells stained with unpurified ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000 dilution (Unpurified)

    Lane 1 : Jurkat lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : 3T3L1 lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 54 kDa
  • Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.

  • Unpurified ab115730 showing positive staining in Normal liver tissue.

  • Unpurified ab115730 showing positive staining in Normal breast tissue.

  • Unpurified ab115730 showing positive staining in Normal colon tissue.

  • Unpurified ab115730 showing positive staining in Kidney carcinoma tissue.

  • Unpurified ab115730 showing negative staining in Skeletal muscle tissue.

  • Unpurified ab115730 showing positive staining in Urinary bladder transitional carcinoma tissue.

  • Unpurified ab115730 showing negative staining in Normal heart tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) 使用論文

This product has been referenced in:
  • Shi S  et al. Metabolic tumor burden is associated with major oncogenomic alterations and serum tumor markers in patients with resected pancreatic cancer. Cancer Lett 360:227-33 (2015). WB ; Human . Read more (PubMed: 25687883) »
  • Qin W  et al. Inhibition of autophagy promotes metastasis and glycolysis by inducing ROS in gastric cancer cells. Oncotarget 6:39839-54 (2015). WB ; Human . Read more (PubMed: 26497999) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 20 µg
Specification Retina
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

投稿 Oct 04 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 20 µg
Specification Mouse brain, heart, liver, muscle
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

投稿 Oct 03 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

投稿 Jul 25 2016

Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 20 µg
Specification HUVEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 25 2016

Thanks for your patience. The specific blocking peptide has been added as catalog number ab202335.



Please let me know if you have any other questions or concerns.

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12.5)
Sample Chinese Hamster Cell lysate - whole cell (ovary)
Specification ovary
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Daniel Wang

Verified customer

投稿 Feb 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Sample Rat Tissue sections (Liver cancer)
Specification Liver cancer
Permeabilization Yes - Wash Buffer from Dako with tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Mar 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with tween
Username

Mr. Rudolf Jung

Verified customer

投稿 Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with tween
Username

Mr. Rudolf Jung

Verified customer

投稿 Apr 16 2013

Thank you for contacting us. The epitipe for the GLU1 antibody ab115730 is intracellular.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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