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Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1 (Peptide available as ab115830.)
Our Abpromise guarantee covers the use of ab652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa. We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab652 staining Glucose Transporter GLUT1 in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA was performed for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at dilution at 1/200. DAPI was used to stain the cell nuclei (blue).