Anti-Glucose Transporter GLUT1 抗体 (ab15309)

製品の概要

  • 製品名Anti-Glucose Transporter GLUT1 antibody
    Glucose Transporter GLUT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose Transporter GLUT1
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • ポジティブ・コントロール
    • HepG2 cells, esophagous and breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 25269858ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 55 kDa. The band may look broad like that for most membrane glycoproteins. A reviewer of another antibody against Glut1, ab652, suggests "do not boil sample before loading as this causes smearing of GLUT-1 bands".
IHC-P 1/200.

ターゲット情報

  • 機能Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • 組織特異性Expressed at variable levels in many human tissues.
  • 関連疾患Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Anti-Glucose Transporter GLUT1 antibody 画像

  • ab15309 staining Glucose Transporter GLUT1 (green) in Human red blood cells tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS-T + 1% PBS) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Red - autofluorescence of erythrocytes.

    See Abreview

  • ab15309 at a 1/100 dilution staining rat cells (neural stem cells from adult subventricular zone) by Immunocytochemistry/Immunofluorescence. The cells were incubated with the antibody for 18 hours and then bound antibody was detected using a Cy3 conjugated Goat anti-rabbit IgG (H + L).

    This image is courtesy of an Abreview submitted by Martin Maurer.

    See Abreview

  • ab15309 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).
  • ab15309 at 1/300 dilution staining GLUT1 in human fetal heart by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed, permeabilized in saponin 0.1% in PBS prior to blocking in 10% serum for 45 minutes at 37°C and then incubated with ab15309 for 1 hour at 37°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/600, was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab15309 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15309, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Glucose Transporter GLUT1 antibody (ab15309) 使用論文

This product has been referenced in:
  • Oprea-Lager DE  et al. [(18)F]fluoromethylcholine as a chemotherapy response read-out in prostate cancer cells. Mol Imaging Biol 17:319-27 (2015). Read more (PubMed: 25407926) »
  • Pertega-Gomes N  et al. A glycolytic phenotype is associated with prostate cancer progression and aggressiveness: a role for monocarboxylate transporters as metabolic targets for therapy. J Pathol 236:517-30 (2015). IHC ; Human . Read more (PubMed: 25875424) »

See all 25 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (canine sarcoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization No
Specification canine sarcoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative 10% NBF
Username

Abcam user community

Verified customer

投稿 Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (melanoma cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization No
Specification melanoma cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative 10% NBF
Username

Abcam user community

Verified customer

投稿 Aug 24 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (H1299 Lung cancer cells)
Specification H1299 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Feb 17 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (human Jurkat (1), mouse 3T3 (2), mouse kidney (3))
Specification human Jurkat (1), mouse 3T3 (2), mouse kidney (3)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 30 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
Sample Human Tissue sections (human red blood cells)
Specification human red blood cells
Permeabilization Yes - Tween-20
Fixative Formaldehyde
Username

Mr. Heiko Locher

Verified customer

投稿 Nov 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount 25 µg
Specification Glioblastoma primary cells
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 07 2013

Thank you for contacting us.

The image which you are referring to was provided to us in an Abreview by a customer. As such do not have all of the specifics of the experiment and cannot know which factors may have lead to the bands showing ...

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Thank you for contacting us.

The two images for WB on the datasheet are under the Abreviews section, so they are results that customers have provided us and not references. One of these reviews does report the band at the "expcetd"...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and underst...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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