Anti-Glucose Transporter 5 GLUT5 抗体 (ab87847)

製品の概要

  • 製品名Anti-Glucose Transporter 5 GLUT5 antibody
    Glucose Transporter 5 GLUT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose Transporter 5 GLUT5
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Glucose Transporter 5 GLUT5.

    (Peptide available as ab97493.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Colon tissue lysate as well as the following whole cell lysates: HeLa; HEK293. IHC-P: Human kidney RCC tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
  • 組織特異性Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
  • 配列類似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Facilitated glucose transporter member 5 antibody
    • Fructose transporter antibody
    • Glucose transporte, kidney antibody
    • glucose transporter like protein 5 antibody
    • Glucose transporter type 5 antibody
    • Glucose transporter type 5 small intestine antibody
    • GLUT 5 antibody
    • GLUT-5 antibody
    • GLUT5 antibody
    • GTR5_HUMAN antibody
    • SLC 2A5 antibody
    • SLC2A5 antibody
    • small intestine antibody
    • solute carrier family 2 (facilitated glucose/fructose transporter), member 5 antibody
    • Solute carrier family 2 antibody
    see all

Anti-Glucose Transporter 5 GLUT5 antibody 画像

  • IHC image of Glucose Transporter 5 GLUT5 staining in human kidney RCC formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87847, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Glucose Transporter 5 GLUT5 antibody (ab87847) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-Glucose Transporter 5 GLUT5 antibody (ab87847) 使用論文

ab87847 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"