Glucose Oxidase Activity Assay Kit (Colorimetric) (ab219924)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate
製品の概要
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製品名
Glucose Oxidase Activity Assay Kit (Colorimetric)
Glucose Oxidase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell Lysate -
アッセイタイプ
Enzyme activity (quantitative) -
全工程の試験時間
0h 20m -
種交差性
交差種: Mammals, Other species -
製品の概要
Glucose Oxidase Activity Assay Kit ab219924 uses a simple colorimetric assay to measure glucose oxidase (GOx) activity in cell and tissue extracts, and biological fluids. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.
The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, leading to an increase in absorbance that can be easily detected at OD575 nm.
Glucose oxidase assay protocol summary:
- add samples and standards to wells
- add glucose oxidase reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37ºC -
特記事項
Glucose oxidase (notatin, GOx, EC 1.1.3.4) is a dimeric enzyme that catalyzes oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 500 tests Assay Buffer 1 x 50ml DMSO 1 x 200µl Glucose 1 vial Glucose Oxidase (100 U) 1 vial Horseradish Peroxidase (HRP) 1 vial Red Dye (light-sensitive) 1 vial -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab219924 は 3 報の論文で使用されています。
- Mohany KM et al. Circulating miR-15b, Annexin A1, procalcitonin and interleukin-6 levels differentiate children with metabolically unhealthy obesity from those with metabolically healthy obesity: A case-control study. Exp Ther Med 23:403 (2022). PubMed: 35637648
- Mohany KM et al. Investigation of the levels of circulating miR-29a, miR-122, sestrin 2 and inflammatory markers in obese children with/without type 2 diabetes: a case control study. BMC Endocr Disord 21:152 (2021). PubMed: 34344352
- Tang W et al. Wet/Sono-Chemical Synthesis of Enzymatic Two-Dimensional MnO2 Nanosheets for Synergistic Catalysis-Enhanced Phototheranostics. Adv Mater 31:e1900401 (2019). PubMed: 30920710