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Abcam's Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (OD570nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
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Glucose (C6H12O6; FW: 180.16) and sucrose (C12H22O11; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase.
|Assay Buffer||WM||1 x 25ml|
|Glucose Enzyme Mix (lyophilized)||Green||1 vial|
|Glucose Probe (in DMSO)||Red||1 x 200µl|
|Invertase (Lyophilized)||Blue||1 vial|
|Sucrose Standard (100 nmol/ul)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab65334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|