Anti-Glucose 6 Phosphate Dehydrogenase 抗体 (ab993)

製品の概要

  • 製品名
    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    Glucose 6 Phosphate Dehydrogenase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • アプリケーション
    適用あり: IHC-Fr, IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
    Database link: P11413

  • ポジティブ・コントロール
    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates;. ICC/IF: HUVEC cells. IHC-Fr: Mouse skeletal muscle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/500.
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • 組織特異性
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • パスウェイ
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • 関連疾患
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • 配列類似性
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • 翻訳後修飾
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

画像

  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 59 kDa


    Exposure time : 30 seconds
  • ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.

    Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

    Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

    Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

See all 14 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 30 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Mouse brain)
Specification
Mouse brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Jun 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Tissue lysate - other (Lung cytosol)
Specification
Lung cytosol
Blocking step
(agent) for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Amit Agarwal

Verified customer

投稿 Jul 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WBCs)
Specification
WBCs
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Verified customer

投稿 Apr 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Leukocytes from bone marrow)
Specification
Leukocytes from bone marrow
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Bone marrow cells)
Specification
Bone marrow cells
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat testis)
Specification
Rat testis
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Abcam user community

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投稿 Nov 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Schizosaccharomyces pombe Cell lysate - other (whole cell lysate)
Loading amount
100 µg
Specification
whole cell lysate
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

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投稿 Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Skin)
Loading amount
15 µg
Specification
Skin
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
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投稿 Apr 24 2007

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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