Goat polyclonal to Glucose 6 Phosphate Dehydrogenase
ab106810 is expected to recognise isoforms NP_000393.4 and NP_001035810.1.
Rat, Sheep, Dog
, corresponding to internal sequence amino acids 308-320 of Human Glucose 6 Phosphate Dehydrogenase (NP_000393.4).
Human Testis, Thyroid and Tonsil lysates; Human testis tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
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Immunogen affinity purified
ab106810 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 59 kDa.Can be blocked with
Glucose 6 Phosphate Dehydrogenase peptide (ab196304).
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 5 - 10 µg/ml. Increasing amount of recombinant G6PD captured by a rabbit antibody. ab106810 used as reporter antibody.
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Isoform Long is found in lymphoblasts, granulocytes and sperm.
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Anemia, non-spherocytic hemolytic, due to G6PD deficiency
Belongs to the glucose-6-phosphate dehydrogenase family.
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
Information by UniProt
Western blot - Glucose 6 Phosphate Dehydrogenase antibody (ab106810)
Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique. Predicted band size: 59 kDa Primary incubation was 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)
ab106810, at 2.5 µg/ml, staining Glucose 6 phosphate dehydrogenase in paraffin-embedded Human testis tissue by Immunohistochemistry using AP staining.
has not yet been referenced specifically in any publications.
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