Anti-glucose-6-phosphatase, catalytic subunit 抗体 (ab93857)

製品の概要

  • 製品名Anti-glucose-6-phosphatase, catalytic subunit antibody
    glucose-6-phosphatase, catalytic subunit 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to glucose-6-phosphatase, catalytic subunit
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cat, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human glucose-6-phosphatase, catalytic subunit.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both HepG2 and HEK293 human whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).

ターゲット情報

  • 機能Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
  • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A) [MIM:232200]. A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia.
  • 配列類似性Belongs to the glucose-6-phosphatase family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW107337 antibody
    • G-6-Pase antibody
    • G6Pase antibody
    • G6Pase-alpha antibody
    • g6pc antibody
    • G6PC_HUMAN antibody
    • G6PT antibody
    • Glucose-6-phosphatase alpha antibody
    • Glucose-6-phosphatase antibody
    • GSD1 antibody
    • GSD1a antibody
    • MGC163350 antibody
    • MGC93613 antibody
    • RP23-281C18.19 antibody
    see all

Anti-glucose-6-phosphatase, catalytic subunit antibody 画像

  • All lanes : Anti-glucose-6-phosphatase, catalytic subunit antibody (ab93857) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,22 kDa,44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-glucose-6-phosphatase, catalytic subunit antibody (ab93857) 使用論文

ab93857 has not yet been referenced specifically in any publications.

Product Wall

Thank you so much for keeping me updated, and I am so happy to hear that the replacement G6PC antibody worked so well! Please let me know if there is anything else that we can do for you, and have a great day!

Thank you for your call today and for letting us know about the trouble with ab83690. It was a pleasure speaking to you.

As we discussed, I'm sending a vial of ab93857 free of charge on the order ***. This is in stock and should arrive tomorr...

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