Anti-glucose-6-phosphatase, catalytic subunit 抗体 (ab83690)

製品の概要

  • 製品名Anti-glucose-6-phosphatase, catalytic subunit antibody
    glucose-6-phosphatase, catalytic subunit 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to glucose-6-phosphatase, catalytic subunit
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Sheep, Human, Pig
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide within residues: NLVFKWILFG QRPYWWVLDT DYYSNTSVPL IKQFPVTCET GPGSPSGHAM , corresponding to N terminal amino acids 72-121 of Human glucose-6-phosphatase, catalytic subunit (NP_000142)

  • ポジティブ・コントロール
    • Fetal lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
  • パスウェイCarbohydrate biosynthesis; gluconeogenesis.
  • 関連疾患Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A) [MIM:232200]. A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia.
  • 配列類似性Belongs to the glucose-6-phosphatase family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW107337 antibody
    • G-6-Pase antibody
    • G6Pase antibody
    • G6Pase-alpha antibody
    • g6pc antibody
    • G6PC_HUMAN antibody
    • G6PT antibody
    • Glucose-6-phosphatase alpha antibody
    • Glucose-6-phosphatase antibody
    • GSD1 antibody
    • GSD1a antibody
    • MGC163350 antibody
    • MGC93613 antibody
    • RP23-281C18.19 antibody
    see all

Anti-glucose-6-phosphatase, catalytic subunit antibody 画像

  • Anti-glucose-6-phosphatase, catalytic subunit antibody (ab83690) at 1 µg/ml + Fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • ab83690 at 5 µg/ml staining glucose-6-phosphatase, catalytic subunit in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
  • ab83690 at 5 µg/ml staining glucose-6-phosphatase, catalytic subunit in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
  • ab83690 at 5 µg/ml staining glucose-6-phosphatase, catalytic subunit in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed, paraffin-embedded sections).
  • Anti-glucose-6-phosphatase, catalytic subunit antibody (ab83690) at 1/2000 dilution + Pig liver tissue whole cell lysate at 30 µg

    Secondary
    IRDye® 800CW-conjugated goat anti-rabbit IgG polyclonal at 1/30000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

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Anti-glucose-6-phosphatase, catalytic subunit antibody (ab83690) 使用論文

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% 37.5:1)
Sample Pig Tissue lysate - whole (Liver)
Specification Liver
Blocking step hermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2014

Thank you for submitting an Abreview for ab836900. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species (sheep), we would like to follow up on thi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Sheep Tissue lysate - whole (Maternal and Fetal Liver)
Loading amount 100 µg
Specification Maternal and Fetal Liver
Gel Running Conditions Reduced Denaturing (10% Tris/Glycine-SDS)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Rob Cordery-Cotter

Verified customer

投稿 Nov 20 2012

Thank you for contacting us. I would like to again apologize for the misinformation which you had received regarding ab83690. I have also had the opportunity to compare the immunogen sequences of our anti-CCR2 antibodies against sheep. Unfortuna...

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Thank you for your phone call.

DISCOUNT CODE: xxx for ab28455, Value: xxx
DISCOUNT CODE: xxx for ab83690, Value: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab28455 and ...

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I am very pleased to hear you would like to test ab28455, ab83690, and ab138003 in sheep. Each code will give you for value listed above off a future order. To redeem this offer, please submit an Abreview for sheep for each antibody and include the cod...

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I wish you the best of luck with your research.
. Preparation of lysate from tissues
1.Dissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases.
2.Place the t...

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Expiration date: 21-12-2012

I am very pleased to hear you would like to accept our offer and test ab96142 and ab83690 in Rat samples. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the ex...

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Thank you so much for keeping me updated, and I am so happy to hear that the replacement G6PC antibody worked so well! Please let me know if there is anything else that we can do for you, and have a great day!

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