The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Abcam recommends using 5% BSA as the blocking agent.
IGRP (islet specific glucose 6 phosphatase catalytic subunit related protein), also known as G6PC2, belongs to the glucose-6-phosphatase catalytic subunit family. These enzymes are part of a multicomponent integral membrane system that catalyses the hydrolysis of glucose-6-phosphate. This hydrolysis is the terminal step in gluconeogenic and glycogenolytic pathways, and allows the release of glucose into the bloodstream. IGRP is found in pancreatic islets and does not exhibit phosphohydrolase activity. However, it is a major target of cell-mediated autoimmunity in diabetes.